Immunology

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This laboratory is well equipped with two Class II biosafety cabinets, three centrifuges, a CO2 incubator, two ELISA plate readers, five refrigerators, a vacuum pump, six -80ºC freezers, 10 liquid nitrogen tanks, three computers, and a seven color flow cytometer.

This laboratory is performing a number of immunological assays including but not limited to peripheral blood/pleural fluid mononuclear cell (PBMC) isolation and storage, flow cytometry for cell phenotypic analysis and polyfunctionality, tissue/cell cultures for cytokine production, ELISAs for detection of cytokines, Elispots for detection of cytokines produced by different cell populations in response to multiple antigens, separation of cell populations by rosettesep, and column separation technique, DNA and RNA preparation from buffy coat and Paxgene preparations and separation and storage of serum, plasma, and buffy coat, for detection of various blood components.